7VSW

Crystal structure of a Fab-like fragment of anti-mesothelin antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	143.900, 143.900, 261.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.170 - 3.000
R-factor	0.2183
Rwork	0.216
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cnr
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.220	3.080
High resolution limit [Å]	3.000	3.000
Rmerge	0.201
Rpim		0.722
Number of reflections	55749	4039
<I/σ(I)>	17.4	2
Completeness [%]	99.9
Redundancy	26.6
CC(1/2)	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4.0 M sodium chloride and 0.1 M HEPES, pH 7.0