7VSP

Crystal strcuture of the tandem B-Box domains of COL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	36.934, 44.478, 99.765
Unit cell angles	90.00, 100.05, 90.00

Refinement procedure

Resolution	22.930 - 2.100
R-factor	0.1799
Rwork	0.179
R-free	0.20020
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.230
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.089	0.052	0.340
Rmeas	0.105	0.061	0.402
Rpim	0.056	0.033	0.213
Total number of observations	124258
Number of reflections	18763	3595	3629
<I/σ(I)>	4.2
Completeness [%]	99.8	99.6	99.6
Redundancy	3.4	3.5	3.4
CC(1/2)		0.994	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	35% 1,4-Dioxane