7VSJ

Crystal structure of MmIMP3-RRM12 in complex with 9-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-23
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97853
Spacegroup name	P 2 21 21
Unit cell lengths	39.811, 62.679, 72.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.780 - 2.380
R-factor	0.2058
Rwork	0.203
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7EYY
RMSD bond length	0.008
RMSD bond angle	1.004
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.470
High resolution limit [Å]	2.380	2.380
Rmerge	0.146	0.731
Rmeas	0.153	0.799
Rpim	0.046	0.313
Number of reflections	7733	763
<I/σ(I)>	18.67	2
Completeness [%]	99.9	99.5
Redundancy	10.3	6
CC(1/2)	0.993	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4000