7VSD

E. coli Ribonuclease HI in complex with one Mg2+ (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	85.363, 85.363, 77.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.690 - 1.700
R-factor	0.1879
Rwork	0.186
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z0u
RMSD bond length	0.016
RMSD bond angle	1.513
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.690	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.129	0.950
Rmeas	0.140	1.049
Rpim	0.056	0.442
Number of reflections	32240	1677
<I/σ(I)>	10.4
Completeness [%]	99.9
Redundancy	11.2
CC(1/2)	0.997	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 30% w/v polyethylene glycol 4,000