7VRR

Crystal structure of Arabidopsis thaliana HDT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	103
Detector technology	CCD
Collection date	2018-01-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979470
Spacegroup name	P 43 21 2
Unit cell lengths	79.961, 79.961, 143.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.110 - 2.950
Rwork	0.224
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vmf
RMSD bond length	0.009
RMSD bond angle	1.315
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.420	3.130
High resolution limit [Å]	2.950	2.950
Rmerge	0.108	0.637
Rmeas	0.120
Number of reflections	10405	1621
<I/σ(I)>	20.2	4.1
Completeness [%]	100.0	100
Redundancy	9.7	10.1
CC(1/2)	0.998	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	301	50 mM MgCl2.6H2O, 0.1M HEPES (pH 7.5), 30% V/V PEG MME 550