7VRG
Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.908, 77.859, 81.972 |
| Unit cell angles | 113.63, 109.90, 95.00 |
Refinement procedure
| Resolution | 43.030 - 2.400 |
| R-factor | 0.2345 |
| Rwork | 0.234 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gqb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.034 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 108457 | 7985 |
| <I/σ(I)> | 4.64 | 0.88 |
| Completeness [%] | 94.6 | 94.2 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.978 | 0.382 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100mM HEPES pH 7.0, 26% (w/v) Jeffamine ED-2001 |
