7VRF
Crystal structure of Oxpecker chromodomain in complex with H3K9me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 |
Unit cell lengths | 31.421, 37.455, 38.757 |
Unit cell angles | 61.23, 88.79, 74.94 |
Refinement procedure
Resolution | 25.720 - 1.700 |
R-factor | 0.1965 |
Rwork | 0.192 |
R-free | 0.23840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u68 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.298 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.233 |
Number of reflections | 15565 | 752 |
<I/σ(I)> | 30.8 | |
Completeness [%] | 95.6 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2M ammonium citrate, 20% (w/v) PEG 3350 |