7VQ6
Structure of a specialized glyoxalase from Gossypium hirsutum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | BRUKER METALJET |
Temperature [K] | 298 |
Detector technology | CMOS |
Collection date | 2020-09-21 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.34 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.255, 49.507, 58.413 |
Unit cell angles | 90.00, 99.38, 90.00 |
Refinement procedure
Resolution | 44.480 - 1.390 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qip |
RMSD bond length | 0.016 |
RMSD bond angle | 2.048 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.480 | 1.490 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.081 | 0.510 |
Number of reflections | 66039 | 2731 |
<I/σ(I)> | 12.8 | 2.09 |
Completeness [%] | 99.8 | |
Redundancy | 6.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | MgAC; Sodium Cacodylate ; PEG 4000. |