7VPW

Crystal structure of Transportin-1 in complex with BAP1 PY-NLS (residues 706-724)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	68.896, 129.688, 170.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.860 - 3.760
R-factor	0.2324
Rwork	0.229
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z5o
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.200	3.880
High resolution limit [Å]	3.760	3.760
Rmerge	0.210
Rpim	0.090
Number of reflections	16059	795
<I/σ(I)>	7.3
Completeness [%]	99.6
Redundancy	6.88
CC(1/2)		0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	0.49 M Sodium phosphate monobasic monohydrate, 0.91 M Sodium phosphate dibasic, pH 6.9