7VPS
Crystal structure of the ARM domain of C. glabrata importin alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2020-08-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.650, 62.628, 154.646 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 31.310 - 2.290 |
| R-factor | 0.2201 |
| Rwork | 0.218 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xgr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Rmerge | 0.116 | 0.540 |
| Number of reflections | 39813 | 1934 |
| <I/σ(I)> | 14.5 | 3.2 |
| Completeness [%] | 97.0 | 95.9 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 0.1 M Tris-HCl pH 7.0, 30% PEG 8000, 0.4 M NaCl |
