7VP9

Crystal structure of human ClpP in complex with ZG111

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	103.806, 97.408, 146.686
Unit cell angles	90.00, 90.63, 90.00

Refinement procedure

Resolution	28.838 - 2.552
R-factor	0.2034
Rwork	0.202
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tg6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.838	30.000	2.640
High resolution limit [Å]	2.550	5.480	2.550
Rmerge		0.054	0.298
Rmeas	0.095	0.059	0.364
Rpim	0.044	0.025	0.204
Number of reflections	90730	9605	8416
<I/σ(I)>	11.6
Completeness [%]	95.2	98.9	89.3
Redundancy	3.8	5.4	2.5
CC(1/2)		0.997	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2M sodium bromide, 20% (w/v) polyethylene glycol 3350