7VP6

Structure of a transcription factor and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	26.628, 51.634, 71.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.260 - 2.570
Rwork	0.184
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vp3
RMSD bond length	0.007
RMSD bond angle	0.812
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.260	2.661
High resolution limit [Å]	2.570	2.570
Rmerge	0.088	0.265
Number of reflections	3381	305
<I/σ(I)>	26.39
Completeness [%]	99.1
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	60% v/v Tacsimate pH 7.0