7VP3

Structure of a transcription factor and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.219, 112.784, 156.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.170 - 3.003
Rwork	0.198
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vp2
RMSD bond length	0.010
RMSD bond angle	1.165
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.170	3.110
High resolution limit [Å]	3.003	3.003
Rmerge	0.260	0.834
Rmeas	0.265
Number of reflections	25799	1836
<I/σ(I)>	19.26
Completeness [%]	93.3
Redundancy	25.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM Sodium citrate/Citric acid pH 5.5, 20% w/v PEG 4000 and 10% v/v 2-Propanol