7VP1

Structure of a transcription factor and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	68.123, 68.123, 210.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.210 - 2.902
R-factor	0.1838
Rwork	0.178
R-free	0.23850
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.941
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	3.006
High resolution limit [Å]	2.902	2.902
Rmerge	0.117	0.615
Rmeas	0.119	0.623
Rpim	0.019	0.099
Number of reflections	6982	653
<I/σ(I)>	38.48
Completeness [%]	99.5
Redundancy	20
CC(1/2)	0.999	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350