7VP0

Crystal structure of P domain from norovirus GI.9 capsid protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	CCD
Collection date	2017-05-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	170.950, 103.550, 76.910
Unit cell angles	90.00, 116.66, 90.00

Refinement procedure

Resolution	48.040 - 2.100
R-factor	0.2389
Rwork	0.237
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rdj
RMSD bond length	0.008
RMSD bond angle	0.956
Data scaling software	XDS (VERSION Nov 1, 2016 BUILT=20170215)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.040	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	134729	21576
<I/σ(I)>	4.44
Completeness [%]	98.1
Redundancy	1.95
CC(1/2)	0.980	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293.15	0.10M BICINE (pH 9), 1.70M Magnesium chloride