7VOU

The crystal structure of human forkhead box protein in complex with DNA 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	47.987, 47.987, 164.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.070 - 3.100
R-factor	0.2269
Rwork	0.221
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cby
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.091	0.045	0.858
Rmeas	0.093	0.046	0.880
Rpim	0.020	0.010	0.192
Total number of observations	83510
Number of reflections	3950	255	185
<I/σ(I)>	9.8
Completeness [%]	100.0	99.6	100
Redundancy	21.1	20	19.5
CC(1/2)		0.998	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bis-tris pH 5.5, 0.15 M NaCl, and 26% PEG 3350