7VNA

drosophlia AHR PAS-B domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-01-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.58
Spacegroup name	P 21 2 21
Unit cell lengths	34.259, 46.787, 81.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.596 - 2.597
R-factor	0.2125
Rwork	0.210
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f1o
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.600	2.650
High resolution limit [Å]	2.590	2.600
Rmerge	0.106	0.578
Number of reflections	4172	133
<I/σ(I)>	17.7
Completeness [%]	95.1
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris-HCl pH 7.0, 0.2 M NaCl, 0.8 M sodium citrate