7VN2

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning ATCACGTGAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-12-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	112.596, 94.206, 102.340
Unit cell angles	90.00, 100.59, 90.00

Refinement procedure

Resolution	35.870 - 2.420
R-factor	0.224
Rwork	0.223
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zd4
RMSD bond length	0.012
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.110	2.510
High resolution limit [Å]	2.420	2.420
Number of reflections	39829	4191
<I/σ(I)>	12.6	2.3
Completeness [%]	99.1
Redundancy	3.4
CC(1/2)	0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM sodium cacodylate pH 6.5, 200 mM potassium chloride, 10 mM magnesium chloride and 10% (w/v) polyethylene glycol (PEG) 4000