7VMZ

Crystal structure of a human Coronavirus 229E antibody C04 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2020-05-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 62
Unit cell lengths	148.215, 148.215, 53.387
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.600 - 2.850
R-factor	0.2496
Rwork	0.248
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6df2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Rmeas	0.289	1.204
Rpim	0.065	0.323
Number of reflections	15842	1570
<I/σ(I)>	16.4
Completeness [%]	99.0	100
Redundancy	18.7	13.2
CC(1/2)	0.976	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15% w/v Polyethylene glycol 3350, 0.01M Magnesium chloride hexahydrate, 0.005M Nickel(II) chloride hexahydrate, 0.1M HEPES pH 7.0