7VMY

Crystal structure of LimF prenyltransferase bound with GSPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	47.280, 47.520, 61.190
Unit cell angles	100.12, 104.69, 96.68

Refinement procedure

Resolution	31.520 - 1.770
R-factor	0.1717
Rwork	0.170
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tty
RMSD bond length	0.013
RMSD bond angle	1.419
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.520	1.830
High resolution limit [Å]	1.770	1.770
Number of reflections	47519	4671
<I/σ(I)>	12.52
Completeness [%]	97.4
Redundancy	6.1
CC(1/2)	0.996	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	Mg(OAc)2, PEG 3350, bis-tris