7VMH
Crystal structure of Arabidopsis thaliana HDT4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2019-07-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97947 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.482, 73.114, 72.580 |
| Unit cell angles | 90.00, 96.86, 90.00 |
Refinement procedure
| Resolution | 44.960 - 1.850 |
| R-factor | 0.1948 |
| Rwork | 0.193 |
| R-free | 0.22843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vmf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.686 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.290 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 38947 | 2203 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 96.9 | 88.6 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.997 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M l-proline, 0.1 m HEPES pH 7.5, 24% W/V PEG 1500 |
