7VMB
Crystal structure of IQSEC1-IQ motif, Sec7PH tandem in complex with calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.010, 81.436, 124.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.522 - 1.998 |
| R-factor | 0.200412394071 |
| Rwork | 0.198 |
| R-free | 0.25231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c0a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.778 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.900 | 2.000 |
| Rmerge | 0.068 | |
| Number of reflections | 46959 | 2345 |
| <I/σ(I)> | 27.36 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.1 | 10.3 |
| CC(1/2) | 0.988 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289.15 | 0.1M HEPES pH 7.5, 4% w/v Polyethylene glycol 8000 |
