7VM8

Crystal structure of the MtDMI1 gating ring

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-10-10
Detector	MAR555 FLAT PANEL
Wavelength(s)	0.9793
Spacegroup name	I 4
Unit cell lengths	110.982, 110.982, 91.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.800 - 3.034
R-factor	0.2626
Rwork	0.260
R-free	0.31930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o6j
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.087	0.039	0.975
Rmeas	0.090	0.040	0.900
Rpim	0.025	0.011	0.289
Number of reflections	14703	1108	1087
<I/σ(I)>	7.1
Completeness [%]	100.0	99.8	100
Redundancy	13.1	12.7	12.3
CC(1/2)		0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	16% (wt/vol) PEG 4000, 0.2M KCl, 0.01M CaCl2, 0.05 M Sodium cacodylate trihydrate pH 5.4