7VKR
Crystal structure of D. melanogaster SAMTOR in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.269, 64.095, 79.274 |
| Unit cell angles | 90.10, 93.27, 96.61 |
Refinement procedure
| Resolution | 43.322 - 2.100 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.23870 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.007 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.322 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.127 | 0.841 |
| Number of reflections | 51008 | 3585 |
| <I/σ(I)> | 9.5 | 1.8 |
| Completeness [%] | 88.5 | 59.6 |
| Redundancy | 4.2 | 4.2 |
| CC(1/2) | 0.995 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.1 M sodium citrate, pH 5.0, and 20% (w/v) PEG 8,000 |
