7VKL

Crystal structure of MmIMP3-KH12 in complex with zipcode RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	37.982, 62.710, 65.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 1.950
R-factor	0.2146
Rwork	0.212
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7EYZ
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.128	1.078
Rmeas	0.133	1.139
Rpim	0.038	0.361
Number of reflections	11985	1169
<I/σ(I)>	23.167	2.2
Completeness [%]	99.8	99.7
Redundancy	12.2	9.5
CC(1/2)	0.992	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M Lithium sulfate monohydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3350