7VKI

ESRP1 qRRM2 in complex with 12mer-RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.94989
Spacegroup name	P 31 2 1
Unit cell lengths	86.118, 86.118, 43.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.250 - 1.650
R-factor	0.1958
Rwork	0.195
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rvj
RMSD bond length	0.007
RMSD bond angle	0.864
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.145	0.334
Rmeas	0.150	0.356
Rpim	0.038	0.118
Number of reflections	22615	2200
<I/σ(I)>	17.429	4.333
Completeness [%]	99.8	98.6
Redundancy	11.8	7
CC(1/2)	0.981	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3350