7VKB

Crystal structure of the a bacterial kinase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97981
Spacegroup name	P 41 21 2
Unit cell lengths	45.220, 45.220, 394.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.870 - 1.820
R-factor	0.2068
Rwork	0.205
R-free	0.24600
Structure solution method	SAD
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.871	1.870
High resolution limit [Å]	1.820	1.820
Rmerge		0.089
Rpim		0.091
Number of reflections	38842	2774
<I/σ(I)>	2
Completeness [%]	100.0	100
Redundancy	15.1
CC(1/2)	1.000	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	0.1M Sodium acetate PH 7.0 12% PEG 3350