7VK9

Crystal structure of xCas9 P411T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97791
Spacegroup name	P 31 2 1
Unit cell lengths	190.023, 190.023, 142.985
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.240 - 2.900
R-factor	0.1947
Rwork	0.194
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cmp
RMSD bond length	0.012
RMSD bond angle	1.201
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.240	3.004
High resolution limit [Å]	2.900	2.900
Rmerge	0.136	0.639
Number of reflections	65982	6547
<I/σ(I)>	32.3
Completeness [%]	99.7
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Sodium Cacodylate pH 7.0, 1M Sodium citrate tribasic dehydrate