7VJS

Human AlkB homolog ALKBH6 in complex with Tris and Ni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	46.374, 64.212, 88.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.724 - 1.792
Rwork	0.197
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3thp
RMSD bond length	0.008
RMSD bond angle	1.467
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.790	1.790
Number of reflections	25643	2131
<I/σ(I)>	31.5
Completeness [%]	99.8
Redundancy	7.1
CC(1/2)	0.994	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000, Tris