7VJ8

SFX structure of archaeal class II CPD photolyase from Methanosarcina mazei in the oxidized state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	293
Detector technology	CCD
Collection date	2017-11-09
Detector	MPCCD
Wavelength(s)	1.7712035
Spacegroup name	P 43 21 2
Unit cell lengths	70.550, 70.550, 245.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.600 - 2.100
R-factor	0.1865
Rwork	0.184
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xry
RMSD bond length	0.003
RMSD bond angle	0.521
Data reduction software	CrystFEL (0.6.3)
Data scaling software	CrystFEL (0.6.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	37362	3630
<I/σ(I)>	7.63	0.56
Completeness [%]	100.0	100
Redundancy	620.8	137.9
CC(1/2)	0.990	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8	277	0.5-0.65 M Li2SO4, 10-15% (W/V) PEG 8000