7VIJ

Crystal structure of USP7-HUBL domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	80.141, 81.429, 149.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.391 - 2.300
R-factor	0.22
Rwork	0.218
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ylm
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	149.550	2.380
High resolution limit [Å]	2.300	2.300
Rmeas	0.051	1.027
Number of reflections	44009	4284
<I/σ(I)>	22	2.5
Completeness [%]	99.4
Redundancy	8.9
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2% v/v Tacsimate pH 7.0, 0.1M imidazole pH 7.0, 8% w/v polyethylene glycol 3350, 5% v/v 2-propanol