7VI8

Crystal structure of ChbG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2020-10-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	54.230, 67.030, 146.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.510 - 1.830
Rwork	0.220
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i5i
RMSD bond length	0.014
RMSD bond angle	1.707
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.510	1.895
High resolution limit [Å]	1.830	1.830
Rmerge	0.107	1.960
Number of reflections	47465	4668
<I/σ(I)>	16.48
Completeness [%]	99.0
Redundancy	12.1
CC(1/2)	0.999	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	15% (v/v) PEG 550 MME, 0.1M MES pH6.5