7VGP
Hen egg lysozyme refolded after denaturation at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.543, 53.445, 71.219 |
| Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
| Resolution | 31.170 - 1.910 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hel |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-2000 (2.3.10) |
| Data scaling software | HKL-2000 (2.3.10) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 17322 | 612 |
| <I/σ(I)> | 15.8 | 0.98 |
| Completeness [%] | 94.8 | 67.4 |
| Redundancy | 3.4 | 2.3 |
| CC(1/2) | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 0.1M sodium acetate, 8%(w/v) sodium chloride, 0.1M cesium chloride |
