7VG8
TYLCV Rep-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-10 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 |
| Unit cell lengths | 67.976, 67.976, 40.433 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.500 - 2.040 |
| R-factor | 0.2241 |
| Rwork | 0.222 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6we0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.500 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 11179 | 1028 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 94.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis-Tris pH 6.5, 28%w/v Polyethylene glyco monomethyl ether 2000 |
