7VEV
Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in complex with MES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-31 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.060, 54.210, 57.012 |
| Unit cell angles | 75.86, 66.90, 87.95 |
Refinement procedure
| Resolution | 26.133 - 1.498 |
| Rwork | 0.167 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.717 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.498 | 1.500 |
| Rmerge | 0.055 | 0.139 |
| Number of reflections | 79704 | 10578 |
| <I/σ(I)> | 27.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG6000 0.2 M Calcium chloride 0.1 M MES |
