7VER

Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in a full open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-18
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	79.961, 84.727, 90.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.848 - 1.699
Rwork	0.180
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vev
RMSD bond length	0.014
RMSD bond angle	1.697
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.848	1.800
High resolution limit [Å]	1.699	1.700
Rmerge	0.045	0.253
Number of reflections	66777	9726
<I/σ(I)>	33.3
Completeness [%]	98.3
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG550MME 0.1 M MES 0.01 M Zinc sulfate 0.0209 mM Polygalacturonic acid sodium