7VEN

Structure of burosumab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	49.510, 66.220, 62.050
Unit cell angles	90.00, 105.99, 90.00

Refinement procedure

Resolution	28.949 - 1.450
R-factor	0.1706
Rwork	0.169
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ggs
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.950	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	88128	8028
<I/σ(I)>	6.3
Completeness [%]	99.7
Redundancy	3.4
CC(1/2)	0.985	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.03M NaF, 0.03M NaBr, 0.03M NaI 0.1M Sodium HEPES, MOPS(acid) pH 7.5 20% v/v PEG500 MME, 10% w/v PEG20000