7VEE

The ligand-free structure of GfsA KSQ-AT didomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	230.763, 230.763, 117.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.530 - 2.550
R-factor	0.1976
Rwork	0.196
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mz0
RMSD bond length	0.005
RMSD bond angle	1.427
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.550	2.550
Rmerge	0.033	0.810
Number of reflections	39110	4388
<I/σ(I)>	29.2	2
Completeness [%]	99.9	100
Redundancy	6.8	7
CC(1/2)	1.000	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	298	sodium malonate, glycerol, sodium chloride, HEPES