7VCQ
structure of viral protein BKRF4 in complex with H3.3-H4-ASF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 286.933, 74.770, 61.191 |
| Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
| Resolution | 28.310 - 3.000 |
| R-factor | 0.2472 |
| Rwork | 0.245 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bnv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.625 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
| Rmerge | 0.123 | 0.051 | 0.659 |
| Rmeas | 0.134 | 0.055 | 0.724 |
| Rpim | 0.052 | 0.021 | 0.294 |
| Total number of observations | 175468 | ||
| Number of reflections | 27290 | 2876 | 2422 |
| <I/σ(I)> | 4.3 | ||
| Completeness [%] | 98.1 | 99.9 | 87.8 |
| Redundancy | 6.4 | 6.4 | 5.5 |
| CC(1/2) | 0.998 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2% Tacsimate pH 7.0; o.1M HEPES pH 7.5; 20% PEG3350 |
