7VCQ

structure of viral protein BKRF4 in complex with H3.3-H4-ASF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	286.933, 74.770, 61.191
Unit cell angles	90.00, 97.50, 90.00

Refinement procedure

Resolution	28.310 - 3.000
R-factor	0.2472
Rwork	0.245
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bnv
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10-2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	3.060
High resolution limit [Å]	2.950	6.350	2.950
Rmerge	0.123	0.051	0.659
Rmeas	0.134	0.055	0.724
Rpim	0.052	0.021	0.294
Total number of observations	175468
Number of reflections	27290	2876	2422
<I/σ(I)>	4.3
Completeness [%]	98.1	99.9	87.8
Redundancy	6.4	6.4	5.5
CC(1/2)		0.998	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2% Tacsimate pH 7.0; o.1M HEPES pH 7.5; 20% PEG3350