7VCP
Frischella perrara beta-fructofuranosidase in complex with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 66.255, 66.255, 463.867 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.800 - 2.000 |
| R-factor | 0.18083 |
| Rwork | 0.179 |
| R-free | 0.21228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vco |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.094 | 0.714 |
| Rpim | 0.022 | 0.166 |
| Number of reflections | 42744 | 6054 |
| <I/σ(I)> | 21.8 | 4.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 18.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2-propanol, PEG 600, PEG 3350, ammonium acetate, HEPES-NaOH |
