7VCL

structure of viral protein BKRF4 in complex with H2A-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	64.474, 78.040, 105.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.853 - 3.200
R-factor	0.2339
Rwork	0.229
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k01
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10-2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.171	0.059	0.747
Rmeas	0.179	0.062	0.790
Rpim	0.051	0.018	0.249
Total number of observations	54863
Number of reflections	4730	276	208
<I/σ(I)>	3.2
Completeness [%]	99.1	99.6	92
Redundancy	11.6	10.7	9.2
CC(1/2)		0.999	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M sodium chloride; 0.1 M HEPES pH 7.5; 35% MPD