7VBS
Structure of the AAA+ ATPase domain of the transcriptional regulator GtrR in Burkholderia cenocepacia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 65 |
Unit cell lengths | 108.678, 108.678, 40.375 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.105 - 1.998 |
R-factor | 0.2004 |
Rwork | 0.198 |
R-free | 0.21750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5exs |
RMSD bond length | 0.003 |
RMSD bond angle | 0.675 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.105 | 2.070 |
High resolution limit [Å] | 1.998 | 1.998 |
Number of reflections | 18704 | 1818 |
<I/σ(I)> | 18.33 | |
Completeness [%] | 99.7 | |
Redundancy | 11.3 | |
CC(1/2) | 1.000 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 11% (w/v) Polyethylene glycol 6000, 100 mM Tris-HCl pH 8.1, 100 mM Ammonium phosphate dibasic |