7V9F
Selenomethionine mutant (L740Sem) of BEN4 domain of protein Bend3 with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 96.831, 96.831, 141.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.153 - 2.500 |
| R-factor | 0.1797 |
| Rwork | 0.177 |
| R-free | 0.22560 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.814 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | |
| Number of reflections | 25183 | 13812 |
| <I/σ(I)> | 38.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 21 | |
| CC(1/2) | 0.082 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM sodium citrate/Citric acid pH 4.0, 200 mM Sodium citrate tribasic, and 17% PEG 3350 |
