7V9E

Crystal structure of a methyl transferase ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.97918
Spacegroup name	P 2 2 21
Unit cell lengths	50.381, 52.507, 99.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.430 - 2.300
R-factor	0.2452
Rwork	0.244
R-free	0.27060
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.715
Data reduction software	ADDREF
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.380	2.430
High resolution limit [Å]	2.300	2.300
Rmerge	0.091	0.622
Rpim	0.045	0.420
Number of reflections	21996	1753
<I/σ(I)>	13
Completeness [%]	98.5	99.8
Redundancy	8.1	5.5
CC(1/2)	0.997	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	0.08 M Potassium chloride, 0.02 M Barium chloride dihydrate, 0.04 M Sodium cacodylate trihydrate pH 6.8, 32% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride