7V7L

Crystal Structure of the Heterodimeric HIF-3a:ARNT Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	65.843, 86.291, 143.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.989 - 2.300
R-factor	0.2063
Rwork	0.204
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zp4
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.299	6.240	2.300
Rmerge	0.067	0.030	1.096
Rmeas	0.070	0.032	1.145
Rpim	0.019	0.009	0.326
Number of reflections	48740	2036	1832
<I/σ(I)>	6
Completeness [%]	99.9	99.6	98.7
Redundancy	13	12.2	11.8
CC(1/2)		1.000	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	Sodium citrate tribasic dihydrate, 2-Propanol, PEG 4000