7V6Q
Crystal structure of sNASP-ASF1A-H3.1-H4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.133, 71.004, 104.394 |
| Unit cell angles | 105.32, 101.99, 95.75 |
Refinement procedure
| Resolution | 14.970 - 3.000 |
| R-factor | 0.1674 |
| Rwork | 0.165 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2io5 7v6p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((1.19.2_4158)) |
| Refinement software | PHENIX ((1.19.2_4158)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.096 | 0.569 |
| Number of reflections | 33846 | 1865 |
| <I/σ(I)> | 13.9 | 2.3 |
| Completeness [%] | 90.7 | |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% (v/v) Tacsimate, pH 5.0 11% (v/v) PEG2000MME |
