7V67
Crystal Structure of Enolase1 from Candida albicans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-01-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 265.204, 62.183, 111.815 |
Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
Resolution | 33.114 - 2.000 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2al2 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.114 | 50.000 | 2.071 |
High resolution limit [Å] | 2.000 | 5.170 | 2.000 |
Rmerge | 0.034 | 0.084 | 0.233 |
Rmeas | 0.049 | 0.094 | 0.233 |
Rpim | 0.034 | 0.041 | 0.330 |
Number of reflections | 116013 | 6965 | 11527 |
<I/σ(I)> | 12.79 | ||
Completeness [%] | 98.9 | 99.7 | 99.86 |
Redundancy | 5.5 | 5.2 | 5.6 |
CC(1/2) | 0.990 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350 |