7V5Y

Crystal structure of hexameric complex of Sa2YoeB-Sa2YefM toxin-antitoxin from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97791
Spacegroup name	P 1 21 1
Unit cell lengths	51.989, 66.984, 82.361
Unit cell angles	90.00, 105.21, 90.00

Refinement procedure

Resolution	48.560 - 2.250
R-factor	0.1827
Rwork	0.180
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a6q
RMSD bond length	0.004
RMSD bond angle	0.558
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	26095	1296
<I/σ(I)>	26.7
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.1 M Ammonium tartrate dibasic pH 7.0, 12% w/v Polyethylene glycol 3350