7V4M

Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	80
Detector technology	CCD
Collection date	2021-04-17
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	75.184, 77.568, 68.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 1.900
R-factor	0.1911
Rwork	0.186
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v4f
RMSD bond length	0.007
RMSD bond angle	0.919
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	16089	1596
<I/σ(I)>	23.6
Completeness [%]	100.0
Redundancy	5.3
CC(1/2)	0.780	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Calcium acetate 0.1M MES pH 6.4 9% V/V PEG 4K 10 mg