7V3C
Crystal structure of NP exonuclease C409A-PCMB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99263 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.851, 58.809, 68.845 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.438 - 1.900 |
R-factor | 0.1896 |
Rwork | 0.187 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v37 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
High resolution limit [Å] | 1.896 | 4.090 | 1.900 |
Rmerge | 0.058 | 0.038 | 0.476 |
Rmeas | 0.061 | 0.040 | 0.514 |
Rpim | 0.019 | 0.012 | 0.188 |
Number of reflections | 40392 | 4256 | 3978 |
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.6 | 98.7 | 99.5 |
Redundancy | 9.2 | 10.7 | 6.7 |
CC(1/2) | 0.999 | 0.719 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350, 2 mM Sodium p-hydroxymercuribenzoate |